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KMID : 0370219850290020090
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Yakhak Hoeji
1985 Volume.29 No. 2 p.90 ~ p.95
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Radioimmunoassay of High-Density Lipoprotein in Japanese Quail Serum
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한병훈/Han BH
한용남/류시용/양현옥/박명환/Han YN/Ryu SY/Yang HO/Park MH
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Abstract
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The development of radioimmunoassay (RIA) for high-density lipoprotein (HDL) in Japanese quail serum will contribute to the screening of drugs acting on cholesterol transport. We have developed a double antibody RIA method for J. quail HDL. The first antibody was raised in rabbit by immunization of HDL isolated by the dextrane sulfate-Mn++ precipitation method. For the preparation of radioiodinated antigen, HDL was further purified by combination of electrophoretic procedure. Using the second antibody raised in goat by rabbit IgG, we have furnished the RIA method for HDL. It showed high specificity and sensitivity of working assay range, 0.1~33mcg HDL/tube. There was no correlation between the radioimmunoassay of HDL and the enzyme assay of HDL-cholesterol.
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KEYWORD
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